8GZN

IgM-var2CSA complex

8GZN の概要

• エントリーDOI: 10.2210/pdb8gzn/pdb
• EMDBエントリー: 34399
• 分子名称: Immunoglobulin heavy constant mu, Erythrocyte membrane protein 1, Immunoglobulin J chain (3 entities in total)
• 機能のキーワード: var2csa, igm, placental malaria, p.falciparum, cell adhesion, cell adhesion-immune system complex, cell adhesion/immune system
• 由来する生物種: Plasmodium falciparum (malaria parasite P. falciparum); 詳細
• タンパク質・核酸の鎖数: 13
• 化学式量合計: 1132409.90

構造登録者

Akhouri, R.R.,Goel, S.,Skoglund, U. (登録日: 2022-09-27, 公開日: 2023-10-25, 最終更新日: 2025-07-02)

主引用文献

Akhouri, R.R.,Goel, S.,Skoglund, U.
Cryo-electron microscopy of IgM-VAR2CSA complex reveals IgM inhibits binding of Plasmodium falciparum to Chondroitin Sulfate A.
Nat Commun, 14:6391-6391, 2023

PubMed Abstract: Placental malaria is caused by Plasmodium falciparum-infected erythrocytes (IEs) adhering to chondroitin sulfate proteoglycans in placenta via VAR2CSA-type PfEMP1. Human pentameric immunoglobulin M (IgM) binds to several types of PfEMP1, including VAR2CSA via its Fc domain. Here, a 3.6 Å cryo-electron microscopy map of the IgM-VAR2CSA complex reveals that two molecules of VAR2CSA bind to the Cµ4 of IgM through their DBL3X and DBL5ε domains. The clockwise and anti-clockwise rotation of the two VAR2CSA molecules on opposite faces of IgM juxtaposes C-termini of both VAR2CSA near the J chain, where IgM creates a wall between both VAR2CSA molecules and hinders its interaction with its receptor. To support this, we show when VAR2CSA is bound to IgM, its staining on IEs as well as binding of IEs to chondroitin sulfate A in vitro is severely compromised.

PubMed: 37828011
DOI: 10.1038/s41467-023-41838-x

実験手法

ELECTRON MICROSCOPY (3.6 Å)