8GXE
PTPN21 FERM PTP complex
Summary for 8GXE
Entry DOI | 10.2210/pdb8gxe/pdb |
Descriptor | Tyrosine-protein phosphatase non-receptor type 21, CHLORIDE ION, ... (4 entities in total) |
Functional Keywords | ptpn21, ferm, phosphotase domain, ptp, ptpd1, hydrolase, protein binding- hydrolase complex, protein binding/ hydrolase |
Biological source | Homo sapiens (human) More |
Total number of polymer chains | 2 |
Total formula weight | 68460.11 |
Authors | Chen, L.,Zheng, Y.Y.,Zhou, C. (deposition date: 2022-09-19, release date: 2023-09-27, Last modification date: 2024-04-17) |
Primary citation | Chen, L.,Qian, Z.,Zheng, Y.,Zhang, J.,Sun, J.,Zhou, C.,Xiao, H. Structural analysis of PTPN21 reveals a dominant-negative effect of the FERM domain on its phosphatase activity. Sci Adv, 10:eadi7404-eadi7404, 2024 Cited by PubMed Abstract: PTPN21 belongs to the four-point-one, ezrin, radixin, moesin (FERM) domain-containing protein tyrosine phosphatases (PTP) and plays important roles in cytoskeleton-associated cellular processes like cell adhesion, motility, and cargo transport. Because of the presence of a WPE loop instead of a WPD loop in the phosphatase domain, it is often considered to lack phosphatase activity. However, many of PTPN21's biological functions require its catalytic activity. To reconcile these findings, we have determined the structures of individual PTPN21 FERM, PTP domains, and a complex between FERM-PTP. Combined with biochemical analysis, we have found that PTPN21 PTP is weakly active and is autoinhibited by association with its FERM domain. Disruption of FERM-PTP interaction results in enhanced ERK activation. The oncogenic HPV18 E7 protein binds to PTP at the same location as PTPN21 FERM, indicating that it may act by displacing the FERM domain from PTP. Our results provide mechanistic insight into PTPN21 and benefit functional studies of PTPN21-mediated processes. PubMed: 38416831DOI: 10.1126/sciadv.adi7404 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (3 Å) |
Structure validation
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