8GUW

Structure of Aurora Kinase A in complex with activator peptide

8GUW の概要

• エントリーDOI: 10.2210/pdb8guw/pdb
• 分子名称: Peptide from Centrosomal protein of 192 kDa,Aurora kinase A, ADENOSINE-5'-DIPHOSPHATE (3 entities in total)
• 機能のキーワード: complex, kinase, cytosolic protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 106623.29

構造登録者

Lee, I.-G.,Park, J. (登録日: 2022-09-13, 公開日: 2023-05-31, 最終更新日: 2023-11-29)

主引用文献

Park, J.G.,Jeon, H.,Shin, S.,Song, C.,Lee, H.,Kim, N.K.,Kim, E.E.,Hwang, K.Y.,Lee, B.J.,Lee, I.G.
Structural basis for CEP192-mediated regulation of centrosomal AURKA.
Sci Adv, 9:eadf8582-eadf8582, 2023

PubMed Abstract: Aurora kinase A (AURKA) performs critical functions in mitosis. Thus, the activity and subcellular localization of AURKA are tightly regulated and depend on diverse factors including interactions with the multiple binding cofactors. How these different cofactors regulate AURKA to elicit different levels of activity at distinct subcellular locations and times is poorly understood. Here, we identified a conserved region of CEP192, the major cofactor of AURKA, that mediates the interaction with AURKA. Quantitative binding studies were performed to map the interactions of a conserved helix (Helix-1) within CEP192. The crystal structure of Helix-1 bound to AURKA revealed a distinct binding site that is different from other cofactor proteins such as TPX2. Inhibiting the interaction between Helix-1 and AURKA in cells led to the mitotic defects, demonstrating the importance of the interaction. Collectively, we revealed a structural basis for the CEP192-mediated AURKA regulation at the centrosome, which is distinct from TPX2-mediated regulation on the spindle microtubule.

PubMed: 37083534
DOI: 10.1126/sciadv.adf8582

実験手法

X-RAY DIFFRACTION (2.7 Å)