8GMO
Bacteriophage T4 capsid shell containing 9DE insertions into the gp23* major capsid protein subunits
This is a non-PDB format compatible entry.
Summary for 8GMO
| Entry DOI | 10.2210/pdb8gmo/pdb |
| EMDB information | 40228 |
| Descriptor | Mature major capsid protein, Mature capsid vertex protein (2 entities in total) |
| Functional Keywords | bacteriophage t4, capsid shell, phage display, decorated capsid, phage head, virus like particle |
| Biological source | Tequatrovirus T4 More |
| Total number of polymer chains | 98 |
| Total formula weight | 4858509.49 |
| Authors | Fokine, A.,Rao, V.B. (deposition date: 2023-03-26, release date: 2023-05-10, Last modification date: 2024-06-19) |
| Primary citation | Zhu, J.,Batra, H.,Ananthaswamy, N.,Mahalingam, M.,Tao, P.,Wu, X.,Guo, W.,Fokine, A.,Rao, V.B. Design of bacteriophage T4-based artificial viral vectors for human genome remodeling. Nat Commun, 14:2928-2928, 2023 Cited by PubMed Abstract: Designing artificial viral vectors (AVVs) programmed with biomolecules that can enter human cells and carry out molecular repairs will have broad applications. Here, we describe an assembly-line approach to build AVVs by engineering the well-characterized structural components of bacteriophage T4. Starting with a 120 × 86 nm capsid shell that can accommodate 171-Kbp DNA and thousands of protein copies, various combinations of biomolecules, including DNAs, proteins, RNAs, and ribonucleoproteins, are externally and internally incorporated. The nanoparticles are then coated with cationic lipid to enable efficient entry into human cells. As proof of concept, we assemble a series of AVVs designed to deliver full-length dystrophin gene or perform various molecular operations to remodel human genome, including genome editing, gene recombination, gene replacement, gene expression, and gene silencing. These large capacity, customizable, multiplex, and all-in-one phage-based AVVs represent an additional category of nanomaterial that could potentially transform gene therapies and personalized medicine. PubMed: 37253769DOI: 10.1038/s41467-023-38364-1 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.9 Å) |
Structure validation
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