8GH6

Bombyx mori R2 retrotransposon initiating target-primed reverse transcription

This is a non-PDB format compatible entry.

Summary for 8GH6

• Entry DOI: 10.2210/pdb8gh6/pdb
• EMDB information: 40033
• Descriptor: Reverse transcriptase-like protein, 28S DNA bottom strand, 3' side, 28S DNA bottom strand, 5' side (priming strand), ... (8 entities in total)
• Functional Keywords: retrotransposon, line, reverse transcriptase, endonuclease, rna binding protein, rna binding protein-rna-dna complex, rna binding protein/rna/dna
• Biological source: Bombyx mori (domestic silkworm); More
• Total number of polymer chains: 5
• Total formula weight: 255812.02

Authors

Wilkinson, M.E.,Zhang, F. (deposition date: 2023-03-09, release date: 2023-04-05, Last modification date: 2023-08-16)

Primary citation

Wilkinson, M.E.,Frangieh, C.J.,Macrae, R.K.,Zhang, F.
Structure of the R2 non-LTR retrotransposon initiating target-primed reverse transcription.
Science, 380:301-308, 2023

PubMed Abstract: Non-long terminal repeat (non-LTR) retrotransposons, or long interspersed nuclear elements (LINEs), are an abundant class of eukaryotic transposons that insert into genomes by target-primed reverse transcription (TPRT). During TPRT, a target DNA sequence is nicked and primes reverse transcription of the retrotransposon RNA. Here, we report the cryo-electron microscopy structure of the R2 non-LTR retrotransposon initiating TPRT at its ribosomal DNA target. The target DNA sequence is unwound at the insertion site and recognized by an upstream motif. An extension of the reverse transcriptase (RT) domain recognizes the retrotransposon RNA and guides the 3' end into the RT active site to template reverse transcription. We used Cas9 to retarget R2 in vitro to non-native sequences, suggesting future use as a reprogrammable RNA-based gene-insertion tool.

PubMed: 37023171
DOI: 10.1126/science.adg7883

Experimental method

ELECTRON MICROSCOPY (3.08 Å)