8GAE

Hsp90 provides platform for CRaf dephosphorylation by PP5

Summary for 8GAE

• Entry DOI: 10.2210/pdb8gae/pdb
• EMDB information: 29895 29949 29957 29973 29976
• Descriptor: Heat shock protein HSP 90-beta, Hsp90 co-chaperone Cdc37, RAF proto-oncogene serine/threonine-protein kinase, ... (9 entities in total)
• Functional Keywords: chaperone, kinase, phosphatase, complex
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 5
• Total formula weight: 294740.59

Authors

Jaime-Garza, M.,Nowotny, C.A.,Coutandin, D.,Wang, F.,Tabios, M.,Agard, D.A. (deposition date: 2023-02-22, release date: 2023-04-26, Last modification date: 2024-10-23)

Primary citation

Jaime-Garza, M.,Nowotny, C.A.,Coutandin, D.,Wang, F.,Tabios, M.,Agard, D.A.
Hsp90 provides a platform for kinase dephosphorylation by PP5.
Nat Commun, 14:2197-2197, 2023

PubMed Abstract: The Hsp90 molecular chaperone collaborates with the phosphorylated Cdc37 cochaperone for the folding and activation of its many client kinases. As with many kinases, the Hsp90 client kinase CRaf is activated by phosphorylation at specific regulatory sites. The cochaperone phosphatase PP5 dephosphorylates CRaf and Cdc37 in an Hsp90-dependent manner. Although dephosphorylating Cdc37 has been proposed as a mechanism for releasing Hsp90-bound kinases, here we show that Hsp90 bound kinases sterically inhibit Cdc37 dephosphorylation indicating kinase release must occur before Cdc37 dephosphorylation. Our cryo-EM structure of PP5 in complex with Hsp90:Cdc37:CRaf reveals how Hsp90 both activates PP5 and scaffolds its association with the bound CRaf to dephosphorylate phosphorylation sites neighboring the kinase domain. Thus, we directly show how Hsp90's role in maintaining protein homeostasis goes beyond folding and activation to include post translationally modifying its client kinases.

PubMed: 37069154
DOI: 10.1038/s41467-023-37659-7

Experimental method

ELECTRON MICROSCOPY (3.3 Å)