8G2Z
48-nm doublet microtubule from Tetrahymena thermophila strain CU428
This is a non-PDB format compatible entry.
Summary for 8G2Z
| Entry DOI | 10.2210/pdb8g2z/pdb |
| EMDB information | 29685 |
| Descriptor | RIB27A, CFAP20, Parkin co-regulated protein PACRGA, ... (49 entities in total) |
| Functional Keywords | cilia, axoneme, doublet microtubule, microtubule inner protein, structural protein |
| Biological source | Tetrahymena thermophila More |
| Total number of polymer chains | 431 |
| Total formula weight | 20357738.10 |
| Authors | Black, C.S.,Kubo, S.,Yang, S.K.,Bui, K.H. (deposition date: 2023-02-06, release date: 2023-06-14, Last modification date: 2024-12-25) |
| Primary citation | Kubo, S.,Black, C.S.,Joachimiak, E.,Yang, S.K.,Legal, T.,Peri, K.,Khalifa, A.A.Z.,Ghanaeian, A.,McCafferty, C.L.,Valente-Paterno, M.,De Bellis, C.,Huynh, P.M.,Fan, Z.,Marcotte, E.M.,Wloga, D.,Bui, K.H. Native doublet microtubules from Tetrahymena thermophila reveal the importance of outer junction proteins. Nat Commun, 14:2168-2168, 2023 Cited by PubMed Abstract: Cilia are ubiquitous eukaryotic organelles responsible for cellular motility and sensory functions. The ciliary axoneme is a microtubule-based cytoskeleton consisting of two central singlets and nine outer doublet microtubules. Cryo-electron microscopy-based studies have revealed a complex network inside the lumen of both tubules composed of microtubule-inner proteins (MIPs). However, the functions of most MIPs remain unknown. Here, we present single-particle cryo-EM-based analyses of the Tetrahymena thermophila native doublet microtubule and identify 42 MIPs. These data shed light on the evolutionarily conserved and diversified roles of MIPs. In addition, we identified MIPs potentially responsible for the assembly and stability of the doublet outer junction. Knockout of the evolutionarily conserved outer junction component CFAP77 moderately diminishes Tetrahymena swimming speed and beat frequency, indicating the important role of CFAP77 and outer junction stability in cilia beating generation and/or regulation. PubMed: 37061538DOI: 10.1038/s41467-023-37868-0 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (4.1 Å) |
Structure validation
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