8FYV

Salmonella enterica serovar Typhimurium chemoreceptor Tsr (taxis to serine and repellents) ligand-binding domain in complex with l-serine

Summary for 8FYV

• Entry DOI: 10.2210/pdb8fyv/pdb
• Descriptor: Methyl-accepting chemotaxis protein, CHLORIDE ION, SERINE, ... (5 entities in total)
• Functional Keywords: chemotaxis, chemoreceptor, chemosensory, tsr, signaling protein
• Biological source: Salmonella enterica subsp. enterica serovar Typhimurium
• Total number of polymer chains: 5
• Total formula weight: 91012.45

Authors

Baylink, A.,Gentry-Lear, Z.,Glenn, S. (deposition date: 2023-01-26, release date: 2024-05-15, Last modification date: 2024-06-12)

Primary citation

Glenn, S.J.,Gentry-Lear, Z.,Shavlik, M.,Harms, M.J.,Asaki, T.J.,Baylink, A.
Bacterial vampirism mediated through taxis to serum.
Elife, 12:-, 2024

PubMed Abstract: Bacteria of the family Enterobacteriaceae are associated with gastrointestinal (GI) bleeding and bacteremia and are a leading cause of death, from sepsis, for individuals with inflammatory bowel diseases. The bacterial behaviors and mechanisms underlying why these bacteria are prone to bloodstream entry remain poorly understood. Herein, we report that clinical isolates of non-typhoidal serovars, , and are rapidly attracted toward sources of human serum. To simulate GI bleeding, we utilized an injection-based microfluidics device and found that femtoliter volumes of human serum are sufficient to induce bacterial attraction to the serum source. This response is orchestrated through chemotaxis and the chemoattractant L-serine, an amino acid abundant in serum that is recognized through direct binding by the chemoreceptor Tsr. We report the first crystal structures of Typhimurium Tsr in complex with L-serine and identify a conserved amino acid recognition motif for L-serine shared among Tsr orthologues. We find Tsr to be widely conserved among Enterobacteriaceae and numerous World Health Organization priority pathogens associated with bloodstream infections. Lastly, we find that Enterobacteriaceae use human serum as a source of nutrients for growth and that chemotaxis and the chemoreceptor Tsr provide a competitive advantage for migration into enterohemorrhagic lesions. We define this bacterial behavior of taxis toward serum, colonization of hemorrhagic lesions, and the consumption of serum nutrients as 'bacterial vampirism', which may relate to the proclivity of Enterobacteriaceae for bloodstream infections.

PubMed: 38820052
DOI: 10.7554/eLife.93178

Experimental method

X-RAY DIFFRACTION (2.2 Å)