8FYV

Salmonella enterica serovar Typhimurium chemoreceptor Tsr (taxis to serine and repellents) ligand-binding domain in complex with l-serine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2022-05-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9765
Spacegroup name	C 1 2 1
Unit cell lengths	125.807, 75.231, 129.588
Unit cell angles	90.00, 116.47, 90.00

Refinement procedure

Resolution	58.000 - 2.200
R-factor	0.2419
Rwork	0.241
R-free	0.25770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.420
Data reduction software	DIALS
Data scaling software	DIALS
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.000	2.260
High resolution limit [Å]	2.200	2.200
Number of reflections	55162	4503
<I/σ(I)>	7.7
Completeness [%]	100.0
Redundancy	18.8
CC(1/2)	0.994	0.838

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.69 M potassium phosphate pH 9.7