8FYA
Cryo-EM structure of Cas1:Cas2-DEDDh:PAM-containing prespacer complex
Summary for 8FYA
Entry DOI | 10.2210/pdb8fya/pdb |
EMDB information | 29561 29562 29563 29564 29565 |
Descriptor | Cas2-DEDDh, Cas1, DNA (28-MER), ... (4 entities in total) |
Functional Keywords | crispr, integrase, crispr adaptation module, pam, prespacer, exonuclease, dna binding protein-dna complex, enzyme, ribonucleoprotein, dna binding protein/dna |
Biological source | Megasphaera More |
Total number of polymer chains | 8 |
Total formula weight | 224120.61 |
Authors | Skopintsev, P.,Tuck, O.T.,Soczek, K.M.,Doudna, J. (deposition date: 2023-01-25, release date: 2023-05-03, Last modification date: 2023-07-05) |
Primary citation | Wang, J.Y.,Tuck, O.T.,Skopintsev, P.,Soczek, K.M.,Li, G.,Al-Shayeb, B.,Zhou, J.,Doudna, J.A. Genome expansion by a CRISPR trimmer-integrase. Nature, 618:855-861, 2023 Cited by PubMed: 37316664DOI: 10.1038/s41586-023-06178-2 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (2.91 Å) |
Structure validation
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