8FW5

Chimeric HsGATOR1-SpGtr-SpLam complex

8FW5 の概要

• エントリーDOI: 10.2210/pdb8fw5/pdb
• EMDBエントリー: 29497
• 分子名称: GATOR complex protein DEPDC5, GUANOSINE-5'-DIPHOSPHATE, ALUMINUM FLUORIDE, ... (12 entities in total)
• 機能のキーワード: mtor complex 1 (mtorc1), rag gtpase, gtr gtpase, lamtor, gator1, nutrient sensing, signaling protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 451146.47

構造登録者

Tettoni, S.D.,Egri, S.B.,Doxsey, D.D.,Ouch, C.,Chang, J.,Song, K.,Xu, C.,Shen, K. (登録日: 2023-01-20, 公開日: 2023-07-26, 最終更新日: 2025-05-14)

主引用文献

Tettoni, S.D.,Egri, S.B.,Doxsey, D.D.,Veinotte, K.,Ouch, C.,Chang, J.Y.,Song, K.,Xu, C.,Shen, K.
Structure of the Schizosaccharomyces pombe Gtr-Lam complex reveals evolutionary divergence of mTORC1-dependent amino acid sensing.
Structure, 31:1065-1076.e5, 2023

PubMed Abstract: mTORC1 is a protein kinase complex that controls cellular growth in response to nutrient availability. Amino acid signals are transmitted toward mTORC1 via the Rag/Gtr GTPases and their upstream regulators. An important regulator is LAMTOR, which localizes Rag/Gtr on the lysosomal/vacuole membrane. In human cells, LAMTOR consists of five subunits, but in yeast, only three or four. Currently, it is not known how variation of the subunit stoichiometry may affect its structural organization and biochemical properties. Here, we report a 3.1 Å-resolution structural model of the Gtr-Lam complex in Schizosaccharomyces pombe. We found that SpGtr shares conserved architecture as HsRag, but the intersubunit communication that coordinates nucleotide loading on the two subunits differs. In contrast, SpLam contains distinctive structural features, but its GTP-specific GEF activity toward SpGtr is evolutionarily conserved. Our results revealed unique evolutionary paths of the protein components of the mTORC1 pathway.

PubMed: 37453417
DOI: 10.1016/j.str.2023.06.012

実験手法

ELECTRON MICROSCOPY (3.08 Å)