8FR8
Structure of Mycobacterium smegmatis Rsh bound to a 70S translation initiation complex
This is a non-PDB format compatible entry.
Summary for 8FR8
| Entry DOI | 10.2210/pdb8fr8/pdb |
| EMDB information | 29397 |
| Descriptor | 50S ribosomal protein L33 2, 50S ribosomal protein L2, 50S ribosomal protein L3, ... (58 entities in total) |
| Functional Keywords | starvation sensing, rela/spot homologue, mycobacterium, 70s initiation complex, translation |
| Biological source | Mycolicibacterium smegmatis MC2 155 More |
| Total number of polymer chains | 58 |
| Total formula weight | 2345278.06 |
| Authors | Majumdar, S.,Sharma, M.R.,Manjari, S.R.,Banavali, N.K.,Agrawal, R.K. (deposition date: 2023-01-06, release date: 2023-05-17, Last modification date: 2024-06-19) |
| Primary citation | Li, Y.,Majumdar, S.,Treen, R.,Sharma, M.R.,Corro, J.,Gamper, H.B.,Manjari, S.R.,Prusa, J.,Banavali, N.K.,Stallings, C.L.,Hou, Y.M.,Agrawal, R.K.,Ojha, A.K. Starvation sensing by mycobacterial RelA/SpoT homologue through constitutive surveillance of translation. Proc.Natl.Acad.Sci.USA, 120:e2302006120-e2302006120, 2023 Cited by PubMed Abstract: The stringent response, which leads to persistence of nutrient-starved mycobacteria, is induced by activation of the RelA/SpoT homolog (Rsh) upon entry of a deacylated-tRNA in a translating ribosome. However, the mechanism by which Rsh identifies such ribosomes in vivo remains unclear. Here, we show that conditions inducing ribosome hibernation result in loss of intracellular Rsh in a Clp protease-dependent manner. This loss is also observed in nonstarved cells using mutations in Rsh that block its interaction with the ribosome, indicating that Rsh association with the ribosome is important for Rsh stability. The cryo-EM structure of the Rsh-bound 70S ribosome in a translation initiation complex reveals unknown interactions between the ACT domain of Rsh and components of the ribosomal L7/L12 stalk base, suggesting that the aminoacylation status of A-site tRNA is surveilled during the first cycle of elongation. Altogether, we propose a surveillance model of Rsh activation that originates from its constitutive interaction with the ribosomes entering the translation cycle. PubMed: 37216503DOI: 10.1073/pnas.2302006120 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.76 Å) |
Structure validation
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