8FO0

The structure of a crystallizable variant of E. coli pyruvate formate-lyase activating enzyme bound to a partially cleaved SAM molecule

Summary for 8FO0

Entry DOI	10.2210/pdb8fo0/pdb
Descriptor	Pyruvate formate-lyase 1-activating enzyme, IRON/SULFUR CLUSTER, S-ADENOSYLMETHIONINE, ... (5 entities in total)
Functional Keywords	radical sam, activase, pfl, oxidoreductase
Biological source	Escherichia coli K-12
Total number of polymer chains	1
Total formula weight	29026.45
Authors	Moody, J.D.,Saxton, A.J.,Galambas, A.,Lawrence, C.M.,Broderick, J.B. (deposition date: 2022-12-29, release date: 2023-05-24, Last modification date: 2023-06-21)
Primary citation	Moody, J.D.,Hill, S.,Lundahl, M.N.,Saxton, A.J.,Galambas, A.,Broderick, W.E.,Lawrence, C.M.,Broderick, J.B. Computational engineering of previously crystallized pyruvate formate-lyase activating enzyme reveals insights into SAM binding and reductive cleavage. J.Biol.Chem., 299:104791-104791, 2023 Cited by PubMed: 37156396 DOI: 10.1016/j.jbc.2023.104791 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.69 Å)

Structure validation