8FO0
The structure of a crystallizable variant of E. coli pyruvate formate-lyase activating enzyme bound to a partially cleaved SAM molecule
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Pyruvate formate-lyase 1-activating enzyme | polymer | 245 | 28237.3 | 1 | UniProt (P0A9N4) Pfam (PF13353) Pfam (PF04055) In PDB | Escherichia coli K-12 | Formate-C-acetyltransferase-activating enzyme 1,PFL-activating enzyme 1 |
2 | A | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 1 | Chemie (SF4) | |||
3 | A | S-ADENOSYLMETHIONINE | non-polymer | 398.4 | 1 | Chemie (SAM) | |||
4 | A | POTASSIUM ION | non-polymer | 39.1 | 1 | Chemie (K) | |||
5 | water | water | 18.0 | 137 | Chemie (HOH) |
Sequence modifications
A: 1 - 245 (UniProt: P0A9N4)
PDB | External Database | Details |
---|---|---|
Glu 1 | Ser 2 | engineered mutation |
Lys 53 | Glu 54 | engineered mutation |
Glu 93 | Ala 94 | engineered mutation |
His 111 | Arg 112 | engineered mutation |
Lys 139 | Gln 140 | engineered mutation |
Arg 151 | Glu 152 | engineered mutation |
Gln 154 | Lys 155 | engineered mutation |
Glu 158 | Asn 159 | engineered mutation |
Glu 222 | Lys 223 | engineered mutation |
Arg 225 | Lys 226 | engineered mutation |
Ala 226 | Lys 227 | engineered mutation |
Arg 230 | Glu 231 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 28237.3 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 789.2 | |
All* | Total formula weight | 29026.4 |