8FKY

Human nucleolar pre-60S ribosomal subunit (State F)

This is a non-PDB format compatible entry.

Summary for 8FKY

• Entry DOI: 10.2210/pdb8fky/pdb
• EMDB information: 29113 29151 29169 29170 29171 29173 29174 29175 29176 29177 29178 29179 29180 29181 29182 29183 29184 29185 29261
• Descriptor: 60S ribosomal protein L12, 60S ribosomal protein L15, 60S ribosomal protein L17, ... (64 entities in total)
• Functional Keywords: pre-60s ribosomal subunit, assembly intermediate, ribosome, nucleoprotein complex
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 59
• Total formula weight: 4041829.58

Authors

Vanden Broeck, A.,Klinge, S. (deposition date: 2022-12-21, release date: 2023-07-12, Last modification date: 2023-07-19)

Primary citation

Vanden Broeck, A.,Klinge, S.
Principles of human pre-60 S biogenesis.
Science, 381:eadh3892-eadh3892, 2023

PubMed Abstract: During the early stages of human large ribosomal subunit (60) biogenesis, an ensemble of assembly factors establishes and fine-tunes the essential RNA functional centers of pre-60 particles by an unknown mechanism. Here, we report a series of cryo-electron microscopy structures of human nucleolar and nuclear pre-60 assembly intermediates at resolutions of 2.5 to 3.2 angstroms. These structures show how protein interaction hubs tether assembly factor complexes to nucleolar particles and how guanosine triphosphatases and adenosine triphosphatase couple irreversible nucleotide hydrolysis steps to the installation of functional centers. Nuclear stages highlight how a conserved RNA-processing complex, the rixosome, couples large-scale RNA conformational changes with pre-ribosomal RNA processing by the RNA degradation machinery. Our ensemble of human pre-60 particles provides a rich foundation with which to elucidate the molecular principles of ribosome formation.

PubMed: 37410842
DOI: 10.1126/science.adh3892

Experimental method

ELECTRON MICROSCOPY (2.67 Å)