8F27
Mirror-image DNA containing 2'-OMe-L-uridine residue
Summary for 8F27
| Entry DOI | 10.2210/pdb8f27/pdb |
| Descriptor | Mirror-image DNA (5'-D(*(0DG)P*(0MU)P*(0DG)P*(0DT)P*(0DA)P*(0DC)P*(0DA)P*(0DC))-3'), SULFATE ION (3 entities in total) |
| Functional Keywords | mirror-image dna, 2'-ome-modification, dna |
| Biological source | synthetic construct |
| Total number of polymer chains | 2 |
| Total formula weight | 4981.30 |
| Authors | Zhang, W.,Dantsu, Y. (deposition date: 2022-11-07, release date: 2023-04-12, Last modification date: 2023-10-25) |
| Primary citation | Dantsu, Y.,Zhang, W. Derivatization of Mirror-Image l-Nucleic Acids with 2'-OMe Modification for Thermal and Structural Stabilization. Chembiochem, 24:e202200764-e202200764, 2023 Cited by PubMed Abstract: To further expand the functionality and enhance the stability of mirror-image nucleic acids as advanced agents for basic research and therapeutic design, we have synthesized 2'-deoxy-2'-methoxy-l-uridine phosphoramidite and incorporated it into l-DNA and l-RNA by solid-phase synthesis quantitatively. We found that the thermostability of l-nucleic acids is dramatically improved after introducing the modifications. Moreover, we successfully crystallized both l-DNA and l-RNA duplexes containing the 2'-OMe modifications and sharing identical sequences. Crystal structure determination and analysis revealed the overall structures of the mirror-image nucleic acids, and for the first time it was possible to interpret the structural deviations caused by 2'-OMe and 2'-OH groups in the oligonucleotides, which are very similar. This novel chemical nucleic acid modification has the potential to be used to design nucleic acid-based therapeutics and materials in the future. PubMed: 36892526DOI: 10.1002/cbic.202200764 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.6 Å) |
Structure validation
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