8F27
Mirror-image DNA containing 2'-OMe-L-uridine residue
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 99 |
Detector technology | CCD |
Collection date | 2022-10-15 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.987 |
Spacegroup name | P 65 |
Unit cell lengths | 46.310, 46.310, 43.520 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.510 - 1.600 |
R-factor | 0.19306 |
Rwork | 0.193 |
R-free | 0.20517 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7moo |
RMSD bond length | 0.026 |
RMSD bond angle | 3.182 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.510 | 1.640 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.044 | 0.559 |
Rmeas | 0.048 | 0.694 |
Rpim | 0.020 | 0.402 |
Number of reflections | 7021 | 470 |
<I/σ(I)> | 22.1 | 2.2 |
Completeness [%] | 99.4 | 93.6 |
Redundancy | 9.8 | 4.8 |
CC(1/2) | 1.000 | 0.880 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.5 M Lithium sulfate, 50 mM TRIS pH 8.5, 5 % w/v Glycerol |