8EO6

Crystal structure of metagenomic class A beta-lactamase precursor LRA-5 in complex with ceftazidime at 2.35 Angstrom resolution

Summary for 8EO6

• Entry DOI: 10.2210/pdb8eo6/pdb
• Descriptor: LRA-5, ACYLATED CEFTAZIDIME (3 entities in total)
• Functional Keywords: beta-lactamase precursor, environmental resistome, soil metagenome, ceftazidime, oxyimino-cephalosporin, antimicrobial protein
• Biological source: uncultured soil bacterium
• Total number of polymer chains: 2
• Total formula weight: 62893.80

Authors

Power, P.,D'Amico Gonzalez, G.,Centron, D.,Gutkind, G.,Handelsman, J.,Klinke, S. (deposition date: 2022-10-02, release date: 2023-10-04)

• Primary citation: D'Amico Gonzalez, G.,Handelsman, J.,Centron, D.,Gutkind, G.,Klinke, S.,Power, P.; Playing beta-Lactamase Evolution: Metagenomic Class A beta-Lactamase LRA-5 is an Inactive Enzyme Capable of Rendering an Active beta-Lactamase by Introduction of Y69Q and V166E Substitutions; to be published,

Experimental method

X-RAY DIFFRACTION (2.35 Å)