8EKG

MHETase variant Thr159Val, Met192Tyr, Tyr252Phe, Tyr503Trp

Summary for 8EKG

• Entry DOI: 10.2210/pdb8ekg/pdb
• Descriptor: Mono(2-hydroxyethyl) terephthalate hydrolase, CALCIUM ION, DI(HYDROXYETHYL)ETHER, ... (6 entities in total)
• Functional Keywords: protein engineering, mhetase, plastic degradation, hydrolase
• Biological source: Ideonella sakaiensis
• Total number of polymer chains: 6
• Total formula weight: 375648.07

Authors

Saunders, J.W.,Frkic, R.L.,Jackson, C.J. (deposition date: 2022-09-20, release date: 2023-10-18, Last modification date: 2024-06-26)

• Primary citation: Saunders, J.W.,Damry, A.M.,Vongsouthi, V.,Spence, M.A.,Frkic, R.L.,Gomez, C.,Yates, P.A.,Matthews, D.S.,Tokuriki, N.,McLeod, M.D.,Jackson, C.J.; Increasing the Soluble Expression and Whole-Cell Activity of the Plastic-Degrading Enzyme MHETase through Consensus Design.; Biochemistry, 2024; PubMed: 38885634; DOI: 10.1021/acs.biochem.4c00165

Experimental method

X-RAY DIFFRACTION (2.65 Å)