8EKG

MHETase variant Thr159Val, Met192Tyr, Tyr252Phe, Tyr503Trp

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0009279	cellular_component	cell outer membrane
A	0042178	biological_process	xenobiotic catabolic process
A	0046872	molecular_function	metal ion binding
A	0052689	molecular_function	carboxylic ester hydrolase activity
A	0071310	biological_process	obsolete cellular response to organic substance
B	0009279	cellular_component	cell outer membrane
B	0042178	biological_process	xenobiotic catabolic process
B	0046872	molecular_function	metal ion binding
B	0052689	molecular_function	carboxylic ester hydrolase activity
B	0071310	biological_process	obsolete cellular response to organic substance
C	0009279	cellular_component	cell outer membrane
C	0042178	biological_process	xenobiotic catabolic process
C	0046872	molecular_function	metal ion binding
C	0052689	molecular_function	carboxylic ester hydrolase activity
C	0071310	biological_process	obsolete cellular response to organic substance
D	0009279	cellular_component	cell outer membrane
D	0042178	biological_process	xenobiotic catabolic process
D	0046872	molecular_function	metal ion binding
D	0052689	molecular_function	carboxylic ester hydrolase activity
D	0071310	biological_process	obsolete cellular response to organic substance
E	0009279	cellular_component	cell outer membrane
E	0042178	biological_process	xenobiotic catabolic process
E	0046872	molecular_function	metal ion binding
E	0052689	molecular_function	carboxylic ester hydrolase activity
E	0071310	biological_process	obsolete cellular response to organic substance
F	0009279	cellular_component	cell outer membrane
F	0042178	biological_process	xenobiotic catabolic process
F	0046872	molecular_function	metal ion binding
F	0052689	molecular_function	carboxylic ester hydrolase activity
F	0071310	biological_process	obsolete cellular response to organic substance

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	ACT_SITE: Acyl-ester intermediate => ECO:0000305|PubMed:30979881

Chain	Residue	Details
A	SEP225
B	SEP225
C	SEP225
D	SEP225
E	SEP225
F	SEP225

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site_id	SWS_FT_FI2
Number of Residues	12
Details	ACT_SITE: Charge relay system => ECO:0000305|PubMed:30979881

Chain	Residue	Details
A	ASP492
E	HIS528
F	ASP492
F	HIS528
A	HIS528
B	ASP492
B	HIS528
C	ASP492
C	HIS528
D	ASP492
D	HIS528
E	ASP492

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site_id	SWS_FT_FI3
Number of Residues	30
Details	BINDING: BINDING => ECO:0000305|PubMed:30979881

Chain	Residue	Details
A	GLY132
B	HIS528
C	GLY132
C	GLU226
C	ARG411
C	SER416
C	HIS528
D	GLY132
D	GLU226
D	ARG411
D	SER416
A	GLU226
D	HIS528
E	GLY132
E	GLU226
E	ARG411
E	SER416
E	HIS528
F	GLY132
F	GLU226
F	ARG411
F	SER416
A	ARG411
F	HIS528
A	SER416
A	HIS528
B	GLY132
B	GLU226
B	ARG411
B	SER416

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site_id	SWS_FT_FI4
Number of Residues	30
Details	BINDING: BINDING => ECO:0000269|PubMed:30979881

Chain	Residue	Details
A	ASP304
B	ILE313
C	ASP304
C	ASP307
C	LEU309
C	ASP311
C	ILE313
D	ASP304
D	ASP307
D	LEU309
D	ASP311
A	ASP307
D	ILE313
E	ASP304
E	ASP307
E	LEU309
E	ASP311
E	ILE313
F	ASP304
F	ASP307
F	LEU309
F	ASP311
A	LEU309
F	ILE313
A	ASP311
A	ILE313
B	ASP304
B	ASP307
B	LEU309
B	ASP311

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