8E7C

Crystal Structure of Porcine Deltacoronavirus (HKU-15) Mpro with Pfizer Intravenous Inhibitor PF-00835231

8E7C の概要

• エントリーDOI: 10.2210/pdb8e7c/pdb
• 分子名称: Main Protease, N-[(2S)-1-({(2S,3S)-3,4-dihydroxy-1-[(3S)-2-oxopyrrolidin-3-yl]butan-2-yl}amino)-4-methyl-1-oxopentan-2-yl]-4-methoxy-1H-indole-2-carboxamide, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: coronavirus, dcov, hku-15, deltacoronavirus, protease, drug resistance, complex, hydrolase, durg discovery, main protease, mpro, substrate complex, pfizer iv compound, pf-00835231, viral protein, viral protein-hydrolase-inhibitor complex, hydrolase-inhibitor complex, hydrolase/inhibitor
• 由来する生物種: Porcine deltacoronavirus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 71857.96

構造登録者

Shaqra, A.M.,Schiffer, C.A. (登録日: 2022-08-23, 公開日: 2023-03-29, 最終更新日: 2024-11-13)

主引用文献

Zvornicanin, S.N.,Shaqra, A.M.,Huang, Q.J.,Ornelas, E.,Moghe, M.,Knapp, M.,Moquin, S.,Dovala, D.,Schiffer, C.A.,Kurt Yilmaz, N.
Crystal Structures of Inhibitor-Bound Main Protease from Delta- and Gamma-Coronaviruses.
Viruses, 15:-, 2023

PubMed Abstract: With the spread of SARS-CoV-2 throughout the globe causing the COVID-19 pandemic, the threat of zoonotic transmissions of coronaviruses (CoV) has become even more evident. As human infections have been caused by alpha- and beta-CoVs, structural characterization and inhibitor design mostly focused on these two genera. However, viruses from the delta and gamma genera also infect mammals and pose a potential zoonotic transmission threat. Here, we determined the inhibitor-bound crystal structures of the main protease (M) from the delta-CoV porcine HKU15 and gamma-CoV SW1 from the beluga whale. A comparison with the apo structure of SW1 M, which is also presented here, enabled the identification of structural arrangements upon inhibitor binding at the active site. The cocrystal structures reveal binding modes and interactions of two covalent inhibitors, PF-00835231 (active form of lufotrelvir) bound to HKU15, and GC376 bound to SW1 M. These structures may be leveraged to target diverse coronaviruses and toward the structure-based design of pan-CoV inhibitors.

PubMed: 36992489
DOI: 10.3390/v15030781

実験手法

X-RAY DIFFRACTION (2.45 Å)