8DZJ

Cryo-EM structure of Acidibacillus sulfuroxidans Cas12f in complex with sgRNA and target DNA

Summary for 8DZJ

Entry DOI	10.2210/pdb8dzj/pdb
EMDB information	27801
Descriptor	OrfB_Zn_ribbon domain-containing protein, target DNA strand, Non-target DNA strand, ... (5 entities in total)
Functional Keywords	crispr, cas12, gene editing, rna binding protein-rna-dna complex, rna binding protein/rna/dna
Biological source	Acidibacillus sulfuroxidans More
Total number of polymer chains	5
Total formula weight	191064.53
Authors	Liu, C.,Zhao, M. (deposition date: 2022-08-07, release date: 2023-07-12, Last modification date: 2023-11-08)
Primary citation	Wu, T.,Liu, C.,Zou, S.,Lyu, R.,Yang, B.,Yan, H.,Zhao, M.,Tang, W. An engineered hypercompact CRISPR-Cas12f system with boosted gene-editing activity. Nat.Chem.Biol., 19:1384-1393, 2023 Cited by PubMed: 37400536 DOI: 10.1038/s41589-023-01380-9 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (2.9 Å)

Structure validation