8DZJ
Cryo-EM structure of Acidibacillus sulfuroxidans Cas12f in complex with sgRNA and target DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | OrfB_Zn_ribbon domain-containing protein | polymer | 446 | 51424.7 | 2 | UniProt (A0A2U3D0N8) Pfam (PF07282) | Acidibacillus sulfuroxidans | AsCas12f |
| 2 | C (C) | target DNA strand | polymer | 42 | 12983.3 | 1 | Homo sapiens | ||
| 3 | D (D) | Non-target DNA strand | polymer | 42 | 12877.3 | 1 | Homo sapiens | ||
| 4 | E (E) | sgRNA | polymer | 193 | 62289.1 | 1 | Acidibacillus sulfuroxidans | ||
| 5 | F (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) |
Sequence modifications
A, B: 1 - 422 (UniProt: A0A2U3D0N8)
| PDB | External Database | Details |
|---|---|---|
| Met -23 | - | expression tag |
| Ala -22 | - | expression tag |
| His -21 | - | expression tag |
| His -20 | - | expression tag |
| His -19 | - | expression tag |
| His -18 | - | expression tag |
| His -17 | - | expression tag |
| His -16 | - | expression tag |
| Ser -15 | - | expression tag |
| Ala -14 | - | expression tag |
| Ala -13 | - | expression tag |
| Leu -12 | - | expression tag |
| Glu -11 | - | expression tag |
| Val -10 | - | expression tag |
| Leu -9 | - | expression tag |
| Phe -8 | - | expression tag |
| Gln -7 | - | expression tag |
| Gly -6 | - | expression tag |
| Pro -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Tyr -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Pro 0 | - | expression tag |
| Ala 225 | Asp 225 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 190999.1 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 65.4 | |
| All* | Total formula weight | 191064.5 |
