8DZJ

Cryo-EM structure of Acidibacillus sulfuroxidans Cas12f in complex with sgRNA and target DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	OrfB_Zn_ribbon domain-containing protein	polymer	446	51424.7	2	UniProt (A0A2U3D0N8) Pfam (PF07282) In PDB	Acidibacillus sulfuroxidans	AsCas12f
2	C	target DNA strand	polymer	42	12983.3	1		Homo sapiens
3	D	Non-target DNA strand	polymer	42	12877.3	1		Homo sapiens
4	E	sgRNA	polymer	193	62289.1	1		Acidibacillus sulfuroxidans
5	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

A, B: 1 - 422 (UniProt: A0A2U3D0N8)

PDB	External Database	Details
Met -23	-	expression tag
Ala -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
Ser -15	-	expression tag
Ala -14	-	expression tag
Ala -13	-	expression tag
Leu -12	-	expression tag
Glu -11	-	expression tag
Val -10	-	expression tag
Leu -9	-	expression tag
Phe -8	-	expression tag
Gln -7	-	expression tag
Gly -6	-	expression tag
Pro -5	-	expression tag
Gly -4	-	expression tag
Tyr -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag
Ala 225	Asp 225	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	190999.1
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	191064.5

*Water molecules are not included.