8DUA

SIV E660.CR54 SOS-2P Env Trimer with ITS92.02

Summary for 8DUA

• Entry DOI: 10.2210/pdb8dua/pdb
• EMDB information: 27631 27718
• Descriptor: Envelope glycoprotein gp41, Envelope glycoprotein gp120, ITS92.02 Heavy Chain, ... (8 entities in total)
• Functional Keywords: siv, its92, hiv-1, quaternary, hiv-2, viral protein-immune system complex, viral protein/immune system
• Biological source: Simian immunodeficiency virus; More
• Total number of polymer chains: 12
• Total formula weight: 387939.90

Authors

Gorman, J.,Kwong, P.D. (deposition date: 2022-07-27, release date: 2022-10-26, Last modification date: 2024-10-30)

Primary citation

Gorman, J.,Wang, C.,Mason, R.D.,Nazzari, A.F.,Welles, H.C.,Zhou, T.,Bess Jr., J.W.,Bylund, T.,Lee, M.,Tsybovsky, Y.,Verardi, R.,Wang, S.,Yang, Y.,Zhang, B.,Rawi, R.,Keele, B.F.,Lifson, J.D.,Liu, J.,Roederer, M.,Kwong, P.D.
Cryo-EM structures of prefusion SIV envelope trimer.
Nat.Struct.Mol.Biol., 29:1080-1091, 2022

PubMed Abstract: Simian immunodeficiency viruses (SIVs) are lentiviruses that naturally infect non-human primates of African origin and seeded cross-species transmissions of HIV-1 and HIV-2. Here we report prefusion stabilization and cryo-EM structures of soluble envelope (Env) trimers from rhesus macaque SIV (SIV) in complex with neutralizing antibodies. These structures provide residue-level definition for SIV-specific disulfide-bonded variable loops (V1 and V2), which we used to delineate variable-loop coverage of the Env trimer. The defined variable loops enabled us to investigate assembled Env-glycan shields throughout SIV, which we found to comprise both N- and O-linked glycans, the latter emanating from V1 inserts, which bound the O-link-specific lectin jacalin. We also investigated in situ SIV-Env trimers on virions, determining cryo-electron tomography structures at subnanometer resolutions for an antibody-bound complex and a ligand-free state. Collectively, these structures define the prefusion-closed structure of the SIV-Env trimer and delineate variable-loop and glycan-shielding mechanisms of immune evasion conserved throughout SIV evolution.

PubMed: 36344847
DOI: 10.1038/s41594-022-00852-1

Experimental method

ELECTRON MICROSCOPY (4.32 Å)