8DQI

Crystal structure of pyrrolysyl-tRNA synthetase from Methanomethylophilus alvus engineered for acridone amino acid (RS1) bound to ATP and acridone after 2- weeks of crystal growth

Summary for 8DQI

Entry DOI	10.2210/pdb8dqi/pdb
Related	8DQG 8DQH 8DQJ
Descriptor	AA_TRNA_LIGASE_II domain-containing protein, ADENOSINE-5'-TRIPHOSPHATE, (2~{S})-2-azanyl-3-(9-oxidanylidene-10~{H}-acridin-2-yl)propanoic acid, ... (7 entities in total)
Functional Keywords	genetic code expansion, methanomethylophilus alvus, pyrrolysyl-trna synthetase, acridone, ligase
Biological source	Candidatus Methanomethylophilus alvus
Total number of polymer chains	2
Total formula weight	63087.23
Authors	Gottfried-Lee, I.,Karplus, P.A.,Mehl, R.A.,Cooley, R.B. (deposition date: 2022-07-19, release date: 2022-12-07, Last modification date: 2023-10-25)
Primary citation	Gottfried-Lee, I.,Perona, J.J.,Karplus, P.A.,Mehl, R.A.,Cooley, R.B. Structures of Methanomethylophilus alvus Pyrrolysine tRNA-Synthetases Support the Need for De Novo Selections When Altering the Substrate Specificity. Acs Chem.Biol., 17:3470-3477, 2022 Cited by PubMed: 36395426 DOI: 10.1021/acschembio.2c00640 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.54 Å)

Structure validation