8DQI
Crystal structure of pyrrolysyl-tRNA synthetase from Methanomethylophilus alvus engineered for acridone amino acid (RS1) bound to ATP and acridone after 2- weeks of crystal growth
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | D, A | AA_TRNA_LIGASE_II domain-containing protein | polymer | 276 | 30690.9 | 2 | UniProt (A0A3G3IHP7) Pfam (PF17759) In PDB | Candidatus Methanomethylophilus alvus | |
2 | D, A | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 2 | Chemie (ATP) | |||
3 | D | (2~{S})-2-azanyl-3-(9-oxidanylidene-10~{H}-acridin-2-yl)propanoic acid | non-polymer | 283.3 | 1 | Chemie (T7Q) | |||
4 | D | TETRAETHYLENE GLYCOL | non-polymer | 194.2 | 1 | Chemie (PG4) | |||
5 | D, A | MAGNESIUM ION | non-polymer | 24.3 | 5 | Chemie (MG) | |||
6 | A | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
7 | water | water | 18.0 | 470 | Chemie (HOH) |
Sequence modifications
D, A: 2 - 275 (UniProt: A0A3G3IHP7)
PDB | External Database | Details |
---|---|---|
Ser 0 | - | expression tag |
Gly 1 | - | expression tag |
Ala 166 | Asn 166 | conflict |
Gly 168 | Val 168 | conflict |
Cys 239 | Trp 239 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 61381.7 | |
Non-Polymers* | Number of molecules | 10 |
Total formula weight | 1705.5 | |
All* | Total formula weight | 63087.2 |