8DNO
Human Brain Aldehyde Dehydrogenase 1 family, member A1
Summary for 8DNO
| Entry DOI | 10.2210/pdb8dno/pdb |
| EMDB information | 27575 |
| Descriptor | Retinal dehydrogenase 1 (1 entity in total) |
| Functional Keywords | human brain, aldh1a1, oxidoreductase |
| Biological source | Homo sapiens (human) |
| Total number of polymer chains | 4 |
| Total formula weight | 219590.36 |
| Authors | Tringides, M.L. (deposition date: 2022-07-11, release date: 2022-11-16, Last modification date: 2025-05-21) |
| Primary citation | Tringides, M.L.,Zhang, Z.,Morgan, C.E.,Su, C.C.,Yu, E.W. A cryo-electron microscopic approach to elucidate protein structures from human brain microsomes. Life Sci Alliance, 6:-, 2023 Cited by PubMed Abstract: We recently developed a "Build and Retrieve" cryo-electron microscopy (cryo-EM) methodology, which is capable of simultaneously producing near-atomic resolution cryo-EM maps for several individual proteins from a heterogeneous, multiprotein sample. Here we report the use of "Build and Retrieve" to define the composition of a raw human brain microsomal lysate. From this sample, we simultaneously identify and solve cryo-EM structures of five different brain enzymes whose functions affect neurotransmitter recycling, iron metabolism, glycolysis, axonal development, energy homeostasis, and retinoic acid biosynthesis. Interestingly, malfunction of these important proteins has been directly linked to several neurodegenerative disorders, such as Alzheimer's, Huntington's, and Parkinson's diseases. Our work underscores the importance of cryo-EM in facilitating tissue and organ proteomics at the atomic level. PubMed: 36450447DOI: 10.26508/lsa.202201724 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.4 Å) |
Structure validation
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