8D2N

Structure of Acidothermus cellulolyticus Cas9 ternary complex (Pre-cleavage)

Summary for 8D2N

• Entry DOI: 10.2210/pdb8d2n/pdb
• Related: 8D2L
• EMDB information: 27142 27143
• Descriptor: CRISPR-associated endonuclease, Csn1 family, Single guide RNA (106-MER), DNA target strand (25-MER), ... (5 entities in total)
• Functional Keywords: cas9, accas9, crispr, rna binding protein, rna binding protein-dna-rna complex, rna binding protein/dna/rna
• Biological source: Acidothermus cellulolyticus 11B; More
• Total number of polymer chains: 4
• Total formula weight: 172759.88

Authors

Rai, J.,Das, A.,Li, H. (deposition date: 2022-05-30, release date: 2023-12-20)

• Primary citation: Das, A.,Rai, J.,Roth, M.O.,Shu, Y.,Medina, M.L.,Barakat, M.R.,Li, H.; Coupled catalytic states and the role of metal coordination in Cas9; Nat Catal, 6:969-977, 2023; DOI: 10.1038/s41929-023-01031-1

Experimental method

ELECTRON MICROSCOPY (2.88 Å)