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8D2N

Structure of Acidothermus cellulolyticus Cas9 ternary complex (Pre-cleavage)

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A
(A)
CRISPR-associated endonuclease, Csn1 familypolymer1138127498.11UniProt (A0LWB3)
Pfam (PF18470)
Pfam (PF01844)
Pfam (PF18541)
Pfam (PF17893)
Pfam (PF17894)
Pfam (PF18525)
Acidothermus cellulolyticus 11B
2B
(B)
Single guide RNA (106-MER)polymer10634230.31Acidothermus cellulolyticus 11B
3C
(X)
DNA target strand (25-MER)polymer257705.01synthetic construct
4D
(D)
DNA non-target strand (5'-D(P*TP*AP*CP*AP*CP*CP*AP*AP*GP*CP*T)-3')polymer113302.21synthetic construct
5E
(A)
MAGNESIUM IONnon-polymer24.31Chemie (MG)
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains4
Total formula weight172735.6
Non-Polymers*Number of molecules1
Total formula weight24.3
All*Total formula weight172759.9
*Water molecules are not included.

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