8D2N
Structure of Acidothermus cellulolyticus Cas9 ternary complex (Pre-cleavage)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | CRISPR-associated endonuclease, Csn1 family | polymer | 1138 | 127498.1 | 1 | UniProt (A0LWB3) Pfam (PF18470) Pfam (PF01844) Pfam (PF18541) Pfam (PF17893) Pfam (PF17894) Pfam (PF18525) In PDB | Acidothermus cellulolyticus 11B | |
2 | B | Single guide RNA (106-MER) | polymer | 106 | 34230.3 | 1 | Acidothermus cellulolyticus 11B | ||
3 | X | DNA target strand (25-MER) | polymer | 25 | 7705.0 | 1 | synthetic construct | ||
4 | D | DNA non-target strand (5'-D(P*TP*AP*CP*AP*CP*CP*AP*AP*GP*CP*T)-3') | polymer | 11 | 3302.2 | 1 | synthetic construct | ||
5 | A | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 172735.6 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 24.3 | |
All* | Total formula weight | 172759.9 |