8CY8

apo form Cryo-EM structure of Campylobacter jejune ketol-acid reductoisommerase crosslinked by Glutaraldehyde

Summary for 8CY8

• Entry DOI: 10.2210/pdb8cy8/pdb
• EMDB information: 27070
• Descriptor: Ketol-acid reductoisomerase (NADP(+)), PENTANEDIAL (2 entities in total)
• Functional Keywords: ketol-acid reductoisomerase, isomerase, enzyme stability, dodecamer, branched-chain amino acid
• Biological source: Campylobacter jejuni
• Total number of polymer chains: 12
• Total formula weight: 429192.83

Authors

Zheng, S.,Guddat, L.W. (deposition date: 2022-05-23, release date: 2023-02-01)

• Primary citation: Lv, Y.,Zheng, S.,Goldenzweig, A.,Liu, F.,Gao, Y.,Yang, X.,Kandale, A.,McGeary, R.P.,Williams, S.,Kobe, B.,Schembri, M.A.,Landsberg, M.J.,Wu, B.,Bruck, T.B.,Sieber, V.,Boden, M.,Rao, Z.,Fleishman, S.J.,Schenk, G.,Guddat, L.W.; Enhancing the Thermal and Kinetic Stability of Ketol-Acid Reductoisomerase, a Central Catalyst of a Cell-Free Enzyme Cascade for the Manufacture of Platform Chemicals; Appl Biosci, 2022; DOI: 10.3390/applbiosci1020011

Experimental method

ELECTRON MICROSCOPY (2.94 Å)