8CX9

Structure of the SARS-COV2 PLpro (C111S) in complex with a dimeric Ubv that inhibits activity by an unusual allosteric mechanism

Summary for 8CX9

• Entry DOI: 10.2210/pdb8cx9/pdb
• Descriptor: Papain-like protease nsp3, Ubiquitin variant UbV.CV2.1, ZINC ION, ... (6 entities in total)
• Functional Keywords: inhibitor complex, allosteric inhibition, viral protein, viral protein-signaling protein complex, viral protein/signaling protein
• Biological source: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV, SARS-CoV-2); More
• Total number of polymer chains: 12
• Total formula weight: 238772.80

Authors

Singer, A.U.,Slater, C.L.,Patel, A.,Russel, R.,Mark, B.L.,Sidhu, S.S. (deposition date: 2022-05-20, release date: 2023-01-25, Last modification date: 2023-10-25)

• Primary citation: van Vliet, V.J.E.,Huynh, N.,Pala, J.,Patel, A.,Singer, A.,Slater, C.,Chung, J.,van Huizen, M.,Teyra, J.,Miersch, S.,Luu, G.K.,Ye, W.,Sharma, N.,Ganaie, S.S.,Russell, R.,Chen, C.,Maynard, M.,Amarasinghe, G.K.,Mark, B.L.,Kikkert, M.,Sidhu, S.S.; Ubiquitin variants potently inhibit SARS-CoV-2 PLpro and viral replication via a novel site distal to the protease active site.; Plos Pathog., 18:e1011065-e1011065, 2022; PubMed: 36548304; DOI: 10.1371/journal.ppat.1011065

Experimental method

X-RAY DIFFRACTION (3.5 Å)