8CX9

Structure of the SARS-COV2 PLpro (C111S) in complex with a dimeric Ubv that inhibits activity by an unusual allosteric mechanism

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0006508	biological_process	proteolysis
A	0008234	molecular_function	cysteine-type peptidase activity
B	0006508	biological_process	proteolysis
B	0008234	molecular_function	cysteine-type peptidase activity
C	0006508	biological_process	proteolysis
C	0008234	molecular_function	cysteine-type peptidase activity
D	0006508	biological_process	proteolysis
D	0008234	molecular_function	cysteine-type peptidase activity

Functional Information from PROSITE/UniProt

site_id	PS00299
Number of Residues	26
Details	UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFsGmlleD

Chain	Residue	Details
E	LYS27-ASP52

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	16
Details	SITE: Interacts with activating enzyme

Chain	Residue	Details
E	ARG54
K	ILE72
L	ARG54
L	ILE72
H	ARG54
H	ILE72
J	ARG54
J	ILE72
E	ILE72
I	ARG54
I	ILE72
F	ARG54
F	ILE72
G	ARG54
G	ILE72
K	ARG54

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	SITE: Essential for function

Chain	Residue	Details
E	THR68
I	THR68
F	THR68
G	THR68
K	THR68
L	THR68
H	THR68
J	THR68

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	MOD_RES: Phosphoserine; by PINK1 => ECO:0000269|PubMed:24660806, ECO:0000269|PubMed:24751536, ECO:0000269|PubMed:24784582, ECO:0000269|PubMed:25474007, ECO:0000269|PubMed:25527291

Chain	Residue	Details
E	SER65
I	SER65
F	SER65
G	SER65
K	SER65
L	SER65
H	SER65
J	SER65

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	MOD_RES: (Microbial infection) ADP-ribosylthreonine => ECO:0000269|PubMed:32330457

Chain	Residue	Details
E	THR66
C	CYS192
C	CYS224
C	CYS226
D	CYS189
D	CYS192
D	CYS224
D	CYS226
I	THR66
F	THR66
G	THR66
K	THR66
L	THR66
H	THR66
J	THR66
C	CYS189

---

site_id	SWS_FT_FI5
Number of Residues	8
Details	MOD_RES: ADP-ribosylglycine => ECO:0000269|PubMed:28525742

Chain	Residue	Details
E	ASN76
I	ASN76
F	ASN76
G	ASN76
K	ASN76
L	ASN76
H	ASN76
J	ASN76

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603

Chain	Residue	Details
E	LYS6
I	LYS6
F	LYS6
G	LYS6
K	LYS6
L	LYS6
H	LYS6
J	LYS6

---

site_id	SWS_FT_FI7
Number of Residues	16
Details	CROSSLNK: Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Chain	Residue	Details
K	ASN76
L	ASN76
H	ASN76
J	ASN76
E	ASN76
I	ASN76
F	ASN76
G	ASN76

---

site_id	SWS_FT_FI8
Number of Residues	16
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144

Chain	Residue	Details
E	LYS11
K	MET48
L	LYS11
L	MET48
H	LYS11
H	MET48
J	LYS11
J	MET48
E	MET48
I	LYS11
I	MET48
F	LYS11
F	MET48
G	LYS11
G	MET48
K	LYS11

---

site_id	SWS_FT_FI9
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305|PubMed:15466860

Chain	Residue	Details
E	LYS27
I	LYS27
F	LYS27
G	LYS27
K	LYS27
L	LYS27
H	LYS27
J	LYS27

---

site_id	SWS_FT_FI10
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:25752573, ECO:0000269|PubMed:25752577, ECO:0000269|PubMed:34239127

Chain	Residue	Details
E	LYS29
I	LYS29
F	LYS29
G	LYS29
K	LYS29
L	LYS29
H	LYS29
J	LYS29

---

site_id	SWS_FT_FI11
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:25752577

Chain	Residue	Details
E	LYS33
I	LYS33
F	LYS33
G	LYS33
K	LYS33
L	LYS33
H	LYS33
J	LYS33

---

site_id	SWS_FT_FI12
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:18719106

Chain	Residue	Details
E	LYS63
I	LYS63
F	LYS63
G	LYS63
K	LYS63
L	LYS63
H	LYS63
J	LYS63

---