8BVE

MoeA2 from Corynebacterium glutamicum

Summary for 8BVE

• Entry DOI: 10.2210/pdb8bve/pdb
• Descriptor: Molybdopterin molybdenumtransferase, SODIUM ION, CITRIC ACID, ... (4 entities in total)
• Functional Keywords: molybdopterin molybdotransferase; cell division; gephyrin-like protein; corynebacteriales, cell cycle
• Biological source: Corynebacterium glutamicum ATCC 13032
• Total number of polymer chains: 2
• Total formula weight: 88697.75

Authors

Martinez, M.,Haouz, A.,Wehenkel, A.M.,Alzari, P.M. (deposition date: 2022-12-03, release date: 2023-02-22, Last modification date: 2024-10-23)

Primary citation

Martinez, M.,Petit, J.,Leyva, A.,Sogues, A.,Megrian, D.,Rodriguez, A.,Gaday, Q.,Ben Assaya, M.,Portela, M.M.,Haouz, A.,Ducret, A.,Grangeasse, C.,Alzari, P.M.,Duran, R.,Wehenkel, A.M.
Eukaryotic-like gephyrin and cognate membrane receptor coordinate corynebacterial cell division and polar elongation.
Nat Microbiol, 8:1896-1910, 2023

PubMed Abstract: The order Corynebacteriales includes major industrial and pathogenic Actinobacteria such as Corynebacterium glutamicum or Mycobacterium tuberculosis. These bacteria have multi-layered cell walls composed of the mycolyl-arabinogalactan-peptidoglycan complex and a polar growth mode, thus requiring tight coordination between the septal divisome, organized around the tubulin-like protein FtsZ, and the polar elongasome, assembled around the coiled-coil protein Wag31. Here, using C. glutamicum, we report the discovery of two divisome members: a gephyrin-like repurposed molybdotransferase (Glp) and its membrane receptor (GlpR). Our results show how cell cycle progression requires interplay between Glp/GlpR, FtsZ and Wag31, showcasing a crucial crosstalk between the divisome and elongasome machineries that might be targeted for anti-mycobacterial drug discovery. Further, our work reveals that Corynebacteriales have evolved a protein scaffold to control cell division and morphogenesis, similar to the gephyrin/GlyR system that mediates synaptic signalling in higher eukaryotes through network organization of membrane receptors and the microtubule cytoskeleton.

PubMed: 37679597
DOI: 10.1038/s41564-023-01473-0

Experimental method

X-RAY DIFFRACTION (2.14 Å)