8BVE

MoeA2 from Corynebacterium glutamicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 43 21 2
Unit cell lengths	94.443, 94.443, 230.564
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.200 - 2.140
R-factor	0.2009
Rwork	0.200
R-free	0.22670
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.604
Data reduction software	XDS (Mar 15, 2019)
Data scaling software	Aimless (1.11.19)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1-4487-000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.200	2.190
High resolution limit [Å]	2.140	2.140
Rpim	0.023	0.352
Number of reflections	58764	4332
<I/σ(I)>	21.1
Completeness [%]	99.7	96.2
Redundancy	26.4	25
CC(1/2)	1.000	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	100 mM Tris pH 8.5, 30% v/v PEG400, 200 mM Na3Cit