8BUY

Rac-binding domain of human MiniBAR

Summary for 8BUY

• Entry DOI: 10.2210/pdb8buy/pdb
• Descriptor: Granule associated Rac and RHOG effector protein 1, 1,2-ETHANEDIOL, SULFATE ION, ... (4 entities in total)
• Functional Keywords: rac effector, rab effector, protein transport
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 2
• Total formula weight: 32046.68

Authors

Pylypenko, O.,Hammich, H.,Houdusse, A. (deposition date: 2022-12-01, release date: 2023-12-13, Last modification date: 2025-06-25)

Primary citation

Serres, M.P.,Shaughnessy, R.,Escot, S.,Hammich, H.,Cuvelier, F.,Salles, A.,Rocancourt, M.,Verdon, Q.,Gaffuri, A.L.,Sourigues, Y.,Malherbe, G.,Velikovsky, L.,Chardon, F.,Sassoon, N.,Tinevez, J.Y.,Callebaut, I.,Formstecher, E.,Houdusse, A.,David, N.B.,Pylypenko, O.,Echard, A.
MiniBAR/GARRE1 is a dual Rac and Rab effector required for ciliogenesis.
Dev.Cell, 58:2477-2494.e8, 2023

PubMed Abstract: Cilia protrude from the cell surface and play critical roles in intracellular signaling, environmental sensing, and development. Reduced actin-dependent contractility and intracellular trafficking are both required for ciliogenesis, but little is known about how these processes are coordinated. Here, we identified a Rac1- and Rab35-binding protein with a truncated BAR (Bin/amphiphysin/Rvs) domain that we named MiniBAR (also known as KIAA0355/GARRE1), which plays a key role in ciliogenesis. MiniBAR colocalizes with Rac1 and Rab35 at the plasma membrane and on intracellular vesicles trafficking to the ciliary base and exhibits fast pulses at the ciliary membrane. MiniBAR depletion leads to short cilia, resulting from abnormal Rac-GTP/Rho-GTP levels and increased acto-myosin-II-dependent contractility together with defective trafficking of IFT88 and ARL13B into cilia. MiniBAR-depleted zebrafish embryos display dysfunctional short cilia and hallmarks of ciliopathies, including left-right asymmetry defects. Thus, MiniBAR is a dual Rac and Rab effector that controls both actin cytoskeleton and membrane trafficking for ciliogenesis.

PubMed: 37875118
DOI: 10.1016/j.devcel.2023.09.010

Experimental method

X-RAY DIFFRACTION (1.6 Å)