8BUY
Rac-binding domain of human MiniBAR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-20 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98008 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 44.714, 65.945, 90.438 |
| Unit cell angles | 90.00, 91.52, 90.00 |
Refinement procedure
| Resolution | 28.330 - 1.600 |
| R-factor | 0.1825 |
| Rwork | 0.181 |
| R-free | 0.21910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.726 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | Arcimboldo |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.944 | 1.625 |
| High resolution limit [Å] | 1.597 | 1.597 |
| Number of reflections | 34818 | 1681 |
| <I/σ(I)> | 14.8 | |
| Completeness [%] | 99.9 | |
| Redundancy | 4.8 | |
| CC(1/2) | 0.999 | 0.796 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | PEG |
