8BRS

Crystal structure of a variant of penicillin G acylase from Bacillaceae i. s. sp. FJAT-27231 with reduced surface entropy and additionally engineered crystal contact.

Summary for 8BRS

Entry DOI	10.2210/pdb8brs/pdb
Descriptor	Penicillin G acylase, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, (R,R)-2,3-BUTANEDIOL, ... (6 entities in total)
Functional Keywords	penicillin, acylase, hydrolase, surface entropy reduction, crystal contact engineering, crystallizability
Biological source	Bacillus sp. FJAT-27231 More
Total number of polymer chains	2
Total formula weight	86659.49
Authors	Wichmann, J.,Mayer, J.,Mattes, H.,Lukat, P.,Blankenfeldt, W.,Biedendieck, R. (deposition date: 2022-11-23, release date: 2023-05-03, Last modification date: 2024-02-07)
Primary citation	Wichmann, J.,Mayer, J.,Hintmann, M.,Lukat, P.,Blankenfeldt, W.,Biedendieck, R. Multistep Engineering of a Penicillin G Acylase for Systematic Improvement of Crystallization Efficiency Cryst.Growth Des., 2023 Cited by DOI: 10.1021/acs.cgd.2c01408 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.2 Å)

Structure validation