8BRS
Crystal structure of a variant of penicillin G acylase from Bacillaceae i. s. sp. FJAT-27231 with reduced surface entropy and additionally engineered crystal contact.
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Penicillin G acylase | polymer | 212 | 24683.8 | 1 | UniProt (A0A0K9H482) In PDB | Bacillus sp. FJAT-27231 | |
2 | B | Penicillin G acylase | polymer | 538 | 61386.9 | 1 | UniProt (A0A0K9H482) Pfam (PF01804) In PDB | Bacillus sp. FJAT-27231 | |
3 | B | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 1 | Chemie (EPE) | |||
4 | B | (R,R)-2,3-BUTANEDIOL | non-polymer | 90.1 | 3 | Chemie (BU3) | |||
5 | B | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
6 | water | water | 18.0 | 842 | Chemie (HOH) |
Sequence modifications
A: 1 - 212 (UniProt: A0A0K9H482)
B: 1 - 538 (UniProt: A0A0K9H482)
PDB | External Database | Details |
---|---|---|
Ala 201 | Lys 225 | engineered mutation |
Ala 202 | Lys 226 | engineered mutation |
Ala 203 | Glu 227 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Ala 135 | Lys 402 | engineered mutation |
Ala 136 | Glu 403 | engineered mutation |
Ala 137 | Lys 404 | engineered mutation |
Ala 403 | Lys 670 | engineered mutation |
Ala 404 | Glu 671 | engineered mutation |
Ala 405 | Glu 672 | engineered mutation |
Leu 408 | Lys 675 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 86070.7 | |
Non-Polymers* | Number of molecules | 6 |
Total formula weight | 588.8 | |
All* | Total formula weight | 86659.5 |