8BP8

SPA of Trypsin untreated Rotavirus TLP spike

This is a non-PDB format compatible entry.

Summary for 8BP8

• Entry DOI: 10.2210/pdb8bp8/pdb
• EMDB information: 16146
• Descriptor: Outer capsid protein VP4, Outer capsid glycoprotein VP7, Intermediate capsid protein VP6, ... (6 entities in total)
• Functional Keywords: rotavirus, spa, viral protein
• Biological source: Rotavirus A; More
• Total number of polymer chains: 31
• Total formula weight: 1534858.09

Authors

Shah, P.N.M.,Stuart, D.I. (deposition date: 2022-11-16, release date: 2023-04-05, Last modification date: 2024-11-06)

Primary citation

Shah, P.N.M.,Gilchrist, J.B.,Forsberg, B.O.,Burt, A.,Howe, A.,Mosalaganti, S.,Wan, W.,Radecke, J.,Chaban, Y.,Sutton, G.,Stuart, D.I.,Boyce, M.
Characterization of the rotavirus assembly pathway in situ using cryoelectron tomography.
Cell Host Microbe, 31:604-615.e4, 2023

PubMed Abstract: Rotavirus assembly is a complex process that involves the stepwise acquisition of protein layers in distinct intracellular locations to form the fully assembled particle. Understanding and visualization of the assembly process has been hampered by the inaccessibility of unstable intermediates. We characterize the assembly pathway of group A rotaviruses observed in situ within cryo-preserved infected cells through the use of cryoelectron tomography of cellular lamellae. Our findings demonstrate that the viral polymerase VP1 recruits viral genomes during particle assembly, as revealed by infecting with a conditionally lethal mutant. Additionally, pharmacological inhibition to arrest the transiently enveloped stage uncovered a unique conformation of the VP4 spike. Subtomogram averaging provided atomic models of four intermediate states, including a pre-packaging single-layered intermediate, the double-layered particle, the transiently enveloped double-layered particle, and the fully assembled triple-layered virus particle. In summary, these complementary approaches enable us to elucidate the discrete steps involved in forming an intracellular rotavirus particle.

PubMed: 36996819
DOI: 10.1016/j.chom.2023.03.004

Experimental method

ELECTRON MICROSCOPY (2.7 Å)