8BOT

Cryo-EM structure of NHEJ supercomplex(trimer)

これはPDB形式変換不可エントリーです。

8BOT の概要

• エントリーDOI: 10.2210/pdb8bot/pdb
• EMDBエントリー: 16145
• 分子名称: DNA repair protein XRCC4, DNA (24-MER), DNA ligase 4, ... (10 entities in total)
• 機能のキーワード: nhej, dna-pk, dna-pkcs, ku70, ku80, xlf, dna repair, dna binding protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 25
• 化学式量合計: 2411092.49

構造登録者

Hardwick, S.W.,Chaplin, A.K. (登録日: 2022-11-15, 公開日: 2023-06-28, 最終更新日: 2023-08-16)

主引用文献

Hardwick, S.W.,Stavridi, A.K.,Chirgadze, D.Y.,De Oliveira, T.M.,Charbonnier, J.B.,Ropars, V.,Meek, K.,Blundell, T.L.,Chaplin, A.K.
Cryo-EM structure of a DNA-PK trimer: higher order oligomerisation in NHEJ.
Structure, 31:895-, 2023

PubMed Abstract: The ability of humans to maintain the integrity of the genome is imperative for cellular survival. DNA double-strand breaks (DSBs) are considered the most critical type of DNA lesion, which can ultimately lead to diseases including cancer. Non-homologous end joining (NHEJ) is one of two core mechanisms utilized to repair DSBs. DNA-PK is a key component in this process and has recently been shown to form alternate long-range synaptic dimers. This has led to the proposal that these complexes can be formed before transitioning to a short-range synaptic complex. Here we present cryo-EM data representing an NHEJ supercomplex consisting of a trimer of DNA-PK in complex with XLF, XRCC4, and DNA Ligase IV. This trimer represents a complex of both long-range synaptic dimers. We discuss the potential role of the trimeric structure, and possible higher order oligomers, as structural intermediates in the NHEJ mechanism, or as functional DNA repair centers.

PubMed: 37311458
DOI: 10.1016/j.str.2023.05.013

実験手法

ELECTRON MICROSCOPY (7.76 Å)