8BO9
NanoLuc-D9R/H57A/K89R mutant complexed with azacoelenterazine bound in intra-barrel catalytic site
Summary for 8BO9
Entry DOI | 10.2210/pdb8bo9/pdb |
Descriptor | Non structural polyprotein, 3-(4-hydroxyphenyl)-8-[(4-hydroxyphenyl)methyl]-5-(phenylmethyl)-1$l^{4},4,7,8-tetrazabicyclo[4.3.0]nona-1(6),2,4-trien-9-one (3 entities in total) |
Functional Keywords | nanoluc-type luciferase with bound substrate analogue - azacoelenterazine, luminescent protein |
Biological source | synthetic construct |
Total number of polymer chains | 3 |
Total formula weight | 62453.25 |
Authors | Marek, M.,Janin, L.Y. (deposition date: 2022-11-15, release date: 2023-09-27, Last modification date: 2023-12-13) |
Primary citation | Nemergut, M.,Pluskal, D.,Horackova, J.,Sustrova, T.,Tulis, J.,Barta, T.,Baatallah, R.,Gagnot, G.,Novakova, V.,Majerova, M.,Sedlackova, K.,Marques, S.M.,Toul, M.,Damborsky, J.,Prokop, Z.,Bednar, D.,Janin, Y.L.,Marek, M. Illuminating the mechanism and allosteric behavior of NanoLuc luciferase. Nat Commun, 14:7864-7864, 2023 Cited by PubMed: 38030625DOI: 10.1038/s41467-023-43403-y PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (3.1 Å) |
Structure validation
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