8BO9
NanoLuc-D9R/H57A/K89R mutant complexed with azacoelenterazine bound in intra-barrel catalytic site
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C | Non structural polyprotein | polymer | 181 | 20392.3 | 3 | UniProt (A0A482LYE4) In PDB | synthetic construct | |
2 | A, B, C | 3-(4-hydroxyphenyl)-8-[(4-hydroxyphenyl)methyl]-5-(phenylmethyl)-1$l^{4},4,7,8-tetrazabicyclo[4.3.0]nona-1(6),2,4-trien-9-one | non-polymer | 425.5 | 3 | Chemie (NSW) | |||
3 | water | water | 18.0 | 20 | Chemie (HOH) |
Sequence modifications
A, B, C: 0 - 169 (UniProt: A0A482LYE4)
PDB | External Database | Details |
---|---|---|
Met -11 | - | initiating methionine |
His -10 | - | expression tag |
His -9 | - | expression tag |
His -8 | - | expression tag |
His -7 | - | expression tag |
His -6 | - | expression tag |
His -5 | - | expression tag |
Ser -4 | - | expression tag |
Asp -3 | - | expression tag |
Asn -2 | - | expression tag |
Met -1 | - | expression tag |
Arg 9 | Asp 81 | engineered mutation |
Ala 57 | His 129 | engineered mutation |
Arg 89 | Lys 161 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 61176.9 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 1276.4 | |
All* | Total formula weight | 62453.3 |