8BMW

SsoCsm

Summary for 8BMW

• Entry DOI: 10.2210/pdb8bmw/pdb
• EMDB information: 16126 16174 16175 16176 16177 2420
• Descriptor: CRISPR-associated small subunit protein (Type III-D), CRISPR-associated Cas7 paralog (Type III-D), RNA (48-MER), ... (9 entities in total)
• Functional Keywords: sulfolobus, type iii crispr, rna binding protein
• Biological source: Saccharolobus solfataricus; More
• Total number of polymer chains: 15
• Total formula weight: 424274.72

Authors

Spagnolo, L.,White, M.F. (deposition date: 2022-11-11, release date: 2023-03-01, Last modification date: 2024-10-23)

Primary citation

Cannone, G.,Kompaniiets, D.,Graham, S.,White, M.F.,Spagnolo, L.
Structure of the Saccharolobus solfataricus type III-D CRISPR effector.
Curr Res Struct Biol, 5:100098-100098, 2023

PubMed Abstract: CRISPR-Cas is a prokaryotic adaptive immune system, classified into six different types, each characterised by a signature protein. Type III systems, classified based on the presence of a Cas10 subunit, are rather diverse multi-subunit assemblies with a range of enzymatic activities and downstream ancillary effectors. The broad array of current biotechnological CRISPR applications is mainly based on proteins classified as Type II, however recent developments established the feasibility and efficacy of multi-protein Type III CRISPR-Cas effector complexes as RNA-targeting tools in eukaryotes. The crenarchaeon has two type III system subtypes (III-B and III-D). Here, we report the cryo-EM structure of the Csm Type III-D complex from (SsoCsm), which uses CRISPR RNA to bind target RNA molecules, activating the Cas10 subunit for antiviral defence. The structure reveals the complex organisation, subunit/subunit connectivity and protein/guide RNA interactions of the SsoCsm complex, one of the largest CRISPR effectors known.

PubMed: 36843655
DOI: 10.1016/j.crstbi.2023.100098

Experimental method

ELECTRON MICROSCOPY (3.5 Å)