8BDU

H33 variant of DoBi scaffold based on PIH1D1 N-terminal domain

Summary for 8BDU

• Entry DOI: 10.2210/pdb8bdu/pdb
• Related: 7AVC
• Descriptor: PIH1 domain-containing protein 1 (1 entity in total)
• Functional Keywords: protein-protein recognition, ribosome display, il-10, cytokine, protein binding
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 2
• Total formula weight: 34148.82

Authors

Kolenko, P.,Mikulecky, P.,Pham, P.N.,Schneider, B. (deposition date: 2022-10-20, release date: 2023-08-16, Last modification date: 2026-03-04)

Primary citation

Kolenko, P.,Mikulecky, P.,Pham, P.N.,Maly, M.,Schneider, B.
Diffraction anisotropy and paired refinement: crystal structure of H33, a protein binder to interleukin 10.
J.Appl.Crystallogr., 56:1261-1266, 2023

PubMed Abstract: Binder H33 is a small protein binder engineered by ribosome display to bind human interleukin 10. Crystals of binder H33 display severe diffraction anisotropy. A set of data files with correction for diffraction anisotropy based on different local signal-to-noise ratios was prepared. Paired refinement was used to find the optimal anisotropic high-resolution diffraction limit of the data: 3.13-2.47 Å. The structure of binder H33 belongs to the 2% of crystal structures with the highest solvent content in the Protein Data Bank.

PubMed: 37555209
DOI: 10.1107/S160057672300479X

Experimental method

X-RAY DIFFRACTION (2.471 Å)