8BDU

H33 variant of DoBi scaffold based on PIH1D1 N-terminal domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	PIH1 domain-containing protein 1	polymer	152	17074.4	2	UniProt (Q9NWS0) Pfam (PF08190) In PDB	Homo sapiens (human)	Nucleolar protein 17 homolog

Sequence modifications

A, B: 23 - 152 (UniProt: Q9NWS0)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 2	-	expression tag
Trp 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
Pro 6	-	expression tag
Gln 7	-	expression tag
Phe 8	-	expression tag
Glu 9	-	expression tag
Lys 10	-	expression tag
Ser 11	-	expression tag
Ala 12	-	expression tag
Ala 13	-	expression tag
Leu 14	-	expression tag
Glu 15	-	expression tag
Val 16	-	expression tag
Leu 17	-	expression tag
Phe 18	-	expression tag
Gln 19	-	expression tag
Gly 20	-	expression tag
Pro 21	-	expression tag
Gly 22	-	expression tag
Tyr 112	Glu 140	engineered mutation
Ser 116	Thr 144	engineered mutation
Ala 119	Arg 147	engineered mutation
Ser 120	Glu 148	engineered mutation
Ser 124	Asp 152	engineered mutation
Arg 127	Asn 155	engineered mutation
Ser 129	Gln 157	engineered mutation
Ser 131	Asn 159	engineered mutation
Ile 133	Glu 161	engineered mutation
Trp 135	Arg 163	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	34148.8
All*	Total formula weight	34148.8

*Water molecules are not included.