8B7W

Complex IL-17A/anti-IL-17A-76

Summary for 8B7W

• Entry DOI: 10.2210/pdb8b7w/pdb
• Descriptor: anti-IL-17A-76, Interleukin-17A, GLYCEROL, ... (6 entities in total)
• Functional Keywords: interleukin 17a in complex with vhh domain, netakimab, crystal structure., immune system, inhibitor, immune system/inhibitor, immune system-inhibitor complex
• Biological source: Lama glama; More
• Total number of polymer chains: 2
• Total formula weight: 27606.14

Authors

Kostareva, O.S.,Svoeglazova, A.,Kolyadenko, I.A.,Dzhus, U.F.,Tishchenko, S.V.,Gabdulkhakov, A.G. (deposition date: 2022-10-03, release date: 2022-12-28, Last modification date: 2024-01-31)

Primary citation

Kostareva, O.,Svoeglazova, A.,Kolyadenko, I.,Nikulin, A.,Evdokimov, S.,Dzhus, U.,Gabdulkhakov, A.,Tishchenko, S.
Two Epitope Regions Revealed in the Complex of IL-17A and Anti-IL-17A V H H Domain.
Int J Mol Sci, 23:-, 2022

PubMed Abstract: Interleukin-17 (IL-17) is a cytokine produced by the Th17 cells. It is involved in chronic inflammation in patients with autoimmune diseases, such as rheumatoid arthritis, systemic lupus erythematosus, multiple sclerosis, and psoriasis. The antibodies targeting IL-17 and/or IL-17R are therapy tools for these diseases. Netakimab is an IL-17A-specific antibody containing a VH derivative domain and a VL variable domain. We have determined the crystal structure of the IL-17A-specific VH domain in complex with IL-17A at 2.85 Å resolution. Certain amino acid residues of the three complementary-determining regions of the VH domain form a network of solvent-inaccessible hydrogen bonds with two epitope regions of IL-17A. The β-turn of IL-17A, which forms the so-called epitope-1, appears to be the main region of IL-17A interaction with the antibody. Contacts formed by the IL-17A mobile C-terminal region residues (epitope-2) further stabilize the antibody-antigen complex.

PubMed: 36499233
DOI: 10.3390/ijms232314904

Experimental method

X-RAY DIFFRACTION (2.85 Å)