8B7O

X-ray structure of Auranofin-human H-chain ferritin

This is a non-PDB format compatible entry.

Summary for 8B7O

• Entry DOI: 10.2210/pdb8b7o/pdb
• Descriptor: Ferritin heavy chain, N-terminally processed, CHLORIDE ION, MAGNESIUM ION, ... (6 entities in total)
• Functional Keywords: ferritin, gold, encapsulation, auranofin, anticancer, transport protein
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 22629.35

Authors

Ferraro, G.,Merlino, A. (deposition date: 2022-09-30, release date: 2022-12-28, Last modification date: 2024-01-31)

Primary citation

Lucignano, R.,Pratesi, A.,Imbimbo, P.,Monti, D.M.,Picone, D.,Messori, L.,Ferraro, G.,Merlino, A.
Evaluation of Auranofin Loading within Ferritin Nanocages.
Int J Mol Sci, 23:-, 2022

PubMed Abstract: Auranofin (AF), a gold(I) compound that is currently used for the treatment of rheumatoid arthritis and is in clinical trials for its promising anticancer activity, was encapsulated within the human H-chain and the horse spleen ferritin nanocages using the alkaline disassembly/reassembly protocol. The aim of the work was to highlight possible differences in their drug loading capacity and efficacy. The drug-loaded ferritins were characterized via UV-vis absorption spectroscopy and inductively coupled plasma-atomic emission spectroscopy to assess AF encapsulation and to define the exact amount of gold atoms trapped in the Ft cavity. The crystal structures allowed us to define the nature of AF interaction with both ferritins and to identify the gold binding sites. Moreover, the biological characterization let us to obtain preliminary information on the cytotoxic effect of AF when bound to the human H-chain.

PubMed: 36430642
DOI: 10.3390/ijms232214162

Experimental method

X-RAY DIFFRACTION (1.17 Å)