8B6S

X-ray structure of the haloalkane dehalogenase HaloTag7 fusion to the green fluorescent protein GFP (ChemoG1) labeled with a chloroalkane tetramethylrhodamine fluorophore substrate

8B6S の概要

• エントリーDOI: 10.2210/pdb8b6s/pdb
• 関連するPDBエントリー: 8B6R 8B6T
• 分子名称: Green fluorescent protein,Haloalkane dehalogenase, [9-[2-carboxy-5-[2-[2-(6-chloranylhexoxy)ethoxy]ethylcarbamoyl]phenyl]-6-(dimethylamino)xanthen-3-ylidene]-dimethyl-azanium, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: haloalkane dehalogenase, halotag, halotag7, self-labeling protein, green fluorescent protein, gfp, fluorophore, tetramethylerhodamine, tmr, hydrolase
• 由来する生物種: Rhodococcus sp.; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 121511.44

構造登録者

Tarnawski, M.,Hellweg, L.,Hiblot, J. (登録日: 2022-09-27, 公開日: 2023-07-26, 最終更新日: 2023-11-15)

• 主引用文献: Hellweg, L.,Edenhofer, A.,Barck, L.,Huppertz, M.C.,Frei, M.S.,Tarnawski, M.,Bergner, A.,Koch, B.,Johnsson, K.,Hiblot, J.; A general method for the development of multicolor biosensors with large dynamic ranges.; Nat.Chem.Biol., 19:1147-1157, 2023; PubMed: 37291200; DOI: 10.1038/s41589-023-01350-1

実験手法

X-RAY DIFFRACTION (1.8 Å)