8B6S
X-ray structure of the haloalkane dehalogenase HaloTag7 fusion to the green fluorescent protein GFP (ChemoG1) labeled with a chloroalkane tetramethylrhodamine fluorophore substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-23 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.99996 |
Spacegroup name | P 1 |
Unit cell lengths | 46.190, 63.710, 89.420 |
Unit cell angles | 93.56, 91.02, 90.85 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.041 | 0.400 |
Number of reflections | 89852 | 13310 |
<I/σ(I)> | 11.99 | 2.12 |
Completeness [%] | 95.3 | 94.3 |
Redundancy | 2 | 2 |
CC(1/2) | 0.998 | 0.752 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.085 M Tris-HCl pH 8.5, 0.17 M sodium acetate, 15% (v/v) glycerol, 27% (m/v) PEG 4000 |