8B6R

X-ray structure of the haloalkane dehalogenase HaloTag7 labeled with a chloroalkane Cyanine3 fluorophore substrate

Summary for 8B6R

• Entry DOI: 10.2210/pdb8b6r/pdb
• Related: 8B6S 8B6T
• Descriptor: Haloalkane dehalogenase, ~{N}-[2-[2-(6-chloranylhexoxy)ethoxy]ethyl]-6-[3,3-dimethyl-2-[(~{E})-3-(1,3,3-trimethylindol-2-ylidene)prop-1-enyl]indol-1-ium-1-yl]hexanamide, CHLORIDE ION, ... (6 entities in total)
• Functional Keywords: haloalkane dehalogenase, halotag, halotag7, self-labeling protein, fluorophore, cyanine 3, cy3, hydrolase
• Biological source: Rhodococcus sp.
• Total number of polymer chains: 1
• Total formula weight: 34317.47

Authors

Tarnawski, M.,Hellweg, L.,Hiblot, J. (deposition date: 2022-09-27, release date: 2023-07-26, Last modification date: 2023-09-06)

• Primary citation: Hellweg, L.,Edenhofer, A.,Barck, L.,Huppertz, M.C.,Frei, M.S.,Tarnawski, M.,Bergner, A.,Koch, B.,Johnsson, K.,Hiblot, J.; A general method for the development of multicolor biosensors with large dynamic ranges.; Nat.Chem.Biol., 19:1147-1157, 2023; PubMed: 37291200; DOI: 10.1038/s41589-023-01350-1

Experimental method

X-RAY DIFFRACTION (1.5 Å)